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Recursion

Tested Stacks: GATK 4.6.1 Samtools 1.21 Nextflow 24.10 Seurat v5.1 Python 3.12
Prerequisites: Basic Linux CLI & Sequencing Formats
📚 Chapter 4 of 4 0% Course Completed

Recursion

Recursion is a programming technique in which a function calls itself to solve a problem. The function continues to call itself until a base condition is met, at which point the function stops calling itself and returns a value.

 

In Python, recursion is implemented using the same function calling syntax as normal functions. However, it is important to ensure that there is a base condition that eventually stops the recursion, otherwise, the program will enter an infinite loop.

 

Here’s an example of how to calculate the nth number in the Fibonacci sequence using recursion in Python:

Pyton
				def fibonacci(n):
    if n == 0:
        return 0
    elif n == 1:
        return 1
    else:
        return fibonacci(n-1) + fibonacci(n-2)

# Example usage
n = 6
fib_n = fibonacci(n)
print("The", n, "th Fibonacci number is", fib_n)

			

In this example, the fibonacci() function recursively calls itself to calculate the nth number in the sequence. The function first checks if n is 0 or 1, as these are the base cases. If n is not 0 or 1, the function recursively calls itself with the arguments (n-1) and (n-2) to calculate the sum of the two preceding numbers in the sequence. The recursion continues until the base cases are reached and the function returns a value.

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Common Pitfalls & Troubleshooting Checklist

  • Java Heap Exhaustion (GATK / Picard): If processes terminate with java.lang.OutOfMemoryError, set your environment variable explicitly: export _JAVA_OPTIONS="-Xmx16g -Djava.io.tmpdir=/tmp".
  • Index Timestamp Mismatch: If tools complain that a BAM index is missing or out of date, regenerate with samtools index -@ 4 sample.bam.
  • Conda Channel Conflicts: Avoid ABI incompatibilities between bioconda and conda-forge by setting strict priority: conda config --set channel_priority strict.
  • Chromosome Naming Contamination: Always verify whether reference FASTA headers use chr1 or 1; divergent naming causes silent alignment drops.
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