Genomics
Transcriptomics
Genome Assembly
DNA Sequencing
Sequencing Technology comparison table
Sequencing Technology | Illumina | Ion Torrent | PacBio | Oxford Nanopore |
Sequencing Method | Sequencing by synthesis | Ion semiconductor sequencing | Single-molecule real-time (SMRT) sequencing | Nanopore sequencing |
Read Length | Short (up to 600 bp) | Short to mid (up to 400 bp) | Long (up to 100 kbp) | Long (up to 2 Mbp) |
Error Rate | Low (0.1-1%) | Low (0.1-1%) | High (15-20%) | High (10-15%) |
Throughput | High (up to 10 Gbp/run) | Mid to high (up to 10 Gbp/run) | Low to mid (up to 1 Gbp/run) | Mid to high (up to 10 Gbp/run) |
Instrument Cost | High | Mid | High | Mid |
Run Cost | High | Mid | Low | Mid to high |
Applications | Whole-genome sequencing, exome sequencing, RNA sequencing, ChIP sequencing, etc. | Amplicon sequencing, targeted sequencing, exome sequencing, RNA sequencing, etc. | De novo assembly, long-read sequencing, structural variation analysis, epigenetic analysis, etc. | Long-read sequencing, de novo assembly, structural variation analysis, epigenetic analysis, etc. |